Experience in Separating Serum Using Disposable Vacuum Blood Collection Tube

1 (Laboratory of Binzhou Municipal People's Hospital, Binzhou 256610) 2 (Binzhou City Hospital, Binzhou 256600) In recent years, the use of disposable vacuum blood collection tubes for the separation of serum has increasingly been widely used clinically. Although its cost is high, there is a lot of advantages such as short separation time, easy operation, and stable chemical composition in the serum compared to conventional methods because of the presence of pre-inert separation gel with hydrophobicity that is inactive to blood. In our hospital, one-time vacuum blood collection tubes manufactured by Japan Taimao Co., Ltd. were used for more than one year. Some of the experiences were summarized as follows: The serum was prepared by routine methods. As blood cells and serum were directly contacted, the metabolism of blood cells consumed the nutrients in the serum. Serum components are constantly changing. If not detected in time, various biochemical indicators in the serum will change, especially the ion indicators will change significantly, thus affecting the accuracy of the results. A disposable vacuum blood collection tube was used to prepare the serum. After centrifugation, the tube was sequentially separated from the top to the bottom: serum, separation gel, and blood cell three layers. The separation gel forms a gel-like barrier between blood cells and serum, blocking and reducing the passage of blood cells using serum components, so that serum components maintain their stability for a long time.

The agent has the characteristics of procoagulant and serum-separating effect on the blood of the injecting person, and has the characteristics that the serum is separated quickly and is much, the temperature is less affected, and the separated serum can be used 100%. Compared with ordinary pipes, water baths are not needed, which saves time and meets the requirements of clinical emergency specimens.

Using a disposable vacuum blood collection tube can reduce the occurrence of hemolysis. Because during the blood collection process, the blood is slowly injected into the human blood vessel along the wall under a small negative pressure, the red blood cells are not easily damaged; at the same time, the silicone coated in the blood vessel can avoid blood cell adhesion and prevent the blood cells from being broken during centrifugation. , not hemolytic. This reduces the influence of hemolysis factors on the test results, and ensures the clinical accuracy of the measurement results.

The one-time vacuum blood collection tube has a one-time safety adhesive seal, and the blood components in the tube and the external environment are always isolated before the blood is collected from the human blood collection tube and the blood serum is separated and detected, thereby preventing the blood from being contaminated; accordingly, The blood can not be sprinkled blood during collection, transportation, numbering and centrifugation, effectively preventing the medical staff from touching the blood sample with fingers, preventing hospital infection and environmental pollution. The main component of disposable vacuum blood collection tube is PET. It is a kind of plastic tube with high hardness. It is not afraid of falling and easy to carry. Even if it is free to fall at a height of 2 meters, it will not spill blood specimens and facilitate the transportation of specimens. And from high-speed centrifugation, no leakage occurs.

Analytical analyzer measurement, compared with the common test tube through the water bath, centrifuge, pipette sample suction to disposable plastic cup and then on the complex process of detection, the operation is simple and reduce the occurrence of errors. In addition, the vacuum blood collection tube allows blood to be collected, serum separated, on-machine testing, blood sample preservation, and waste disposal all in the same tube, reducing disposable plastic product waste, and protecting the internal and external environment of the laboratory. Increased serum quality before extension.

It is worth noting that if the specimens are taken from the blood to the serum for separation, if the time is too short or the centrifugation time is too short, there will be a cloudy fibrin group on the separating gel. If it is not noticed, it may cause the auto-analyzer. Absorption is inaccurate, affecting the authenticity of the test results, and it may even clog the automated analyzer, causing serious consequences.

If this happens, the sample can be centrifuged again or the serum can be directly poured into disposable plastic cups to obtain clear serum.